Tutorial

Tentative annotation

Criteria to annotate

BLAST Cutoff	Tentative Annotation
Identity >= 80% AND E_Value <= 1e-40 AND Coverage >= 80%	Homologue to Accession/Definition of Protein
(E_Value <= 1e-40 AND (Identity < 90% OR Coverage < 80%)) OR 1e-40 < E_Value <= 1e-30	Similar to Accession/Definition of Protein
1e-30 < E_Value <= 1e-5	Weak similar to Accession/Definition of Protein

Coverage

Based on the Figure above, we define the calculation of full length coverage as below:
Full Length Coverage = [(The sum of the each hit - overlap)/The length of the subject] x 100
Using the above example, the length of the target = 15,
the sum of the length of the hits = (7 + 5 + 3) = 15 and the overlap = 1
Thus, the full length coverage = [((7 + 5 + 3)-1)/15] x100 = 93.3%