Orchidstra 2.0 - A Transcriptomics Resource for the Orchid Family
 

Tutorial

  • Tentative annotation
    • Criteria to annotate
    • BLAST CutoffTentative Annotation
      Identity >= 90% and E_Value <= 1e-40Homologue to Accession/Definition of Protein
      (Identity < 90% and E_Value <= 1e-40) or 1e-40 < E_Value <= 1e-30Similar to Accession/Definition of Protein
      1e-30 < E_Value <= 1e-20 Weak similar to Accession/Definition of Protein
      1e-20 < E_value <= 1e-10 Putative Protein to Accession/Definition of Protein
  • Coverage
    • Based on the Figure above, we define the calculation of full length coverage as below:
      Full Length Coverage = [(The sum of the each hit - overlap)/The length of the subject] x 100
      Using the above example, the length of the target = 15,
      the sum of the length of the hits = (7 + 5 + 3) = 15 and the overlap = 1
      Thus, the full length coverage = [((7 + 5 + 3)-1)/15] x100 = 93.3%